We use Feather Microtome Blades S35L as our new blades.Use the new blade previously used for main cutting as the new blade for rough cutting.Fine-adjust the paraffin blocks to make them horizontal to the blade.Fix the paraffin blocks to the microtome.After this step, mounting the paraffin blocks to the stage is complete.Extra paraffin can be melted and added as needed when there are gaps between the paraffin blocks and stage or areas where the electric soldering iron may come into contact with the sample because there is only a thin layer of paraffin surrounding the paraffin blocks.It is not necessary to melt the paraffin on the bottom surface of the paraffin blocks. The paraffin block is fixed to the stage by melting the paraffin coating the stage and paraffin blocks, which fuses them together.After the paraffin block is placed on the stage, melt the paraffin surrounding the paraffin block with an electric soldering iron.First, using paraffin, coat the surface of the metal specimen stage.Slice off the excess paraffin with a fish-slicing knife to fit the section onto a glass slide. Measure paraffin block sizes with a ruler and determine the appropriate glass slide sizes.Prepare a set for mounting the paraffin blocks onto a metal specimen stage, including a block, electric soldering iron, ruler, fish-slicing knife, and air cleaner.
Use a microtome to cut tissue blocks which are not mounted on a wooden block.
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